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. 2004 May 20;23(11):2235–2245. doi: 10.1038/sj.emboj.7600237

Figure 4.

Figure 4

GSK3 mediates reduced kinesin-driven motility due to CDK5 inhibition. (A) Co-perfusion of 0.5 mM CREBp and 5 μM Olo abrogates the effects of Olo on FAT. (B) Average transport rates are shown for anterograde and retrograde FAT with control buffer (buf), 1 mM CREBp (cr), 5 μM Olo (ol) and 5 μM Olo plus CREBp (crol). Note that CREBp blocks the effect of Olo on anterograde FAT. CREBp alone did not affect either direction of transport. Asterisk denotes P<0.001. (C) Inhibiting CDK5 in cortical neurons reduces the amount of kinesin associated with microsomes (MBOs) but not total kinesin (Total). Representative immunoblots of KHC with H2 and fluorescent secondary antibodies are shown. The Y-axis shows pixel values from Typhoon scans.