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Gelatinase activity. (a)–(d) Florescent micrographs of control (a) and (b), and diabetic (c) and (d) aorta cryosections. Green fluorescence (FITC) indicates areas of gelatinase activity whilst blue fluorescence (DAPI) marks DNA in cell nuclei. (e) Box and whisker plot showing difference in gelatinase activity. (f) This activity is localized to the interlamellar regions which contain vascular smooth muscle cells (as visualized by DAPI). Scales bars 50 μm (a–d) and 20 μm (f).
