Fig. 3.

Immunolocalization of GAGs across bovine corneal tissue. (A) High-sulphated KS and, (B) lesser-sulphated KS were labelled with 5D4 and 1B4 antibodies, respectively. 2B6 antibody was used to specifically recognize: (C) DS GAG chains after pre-treatment with chondroitinase B; (D) CS GAG chains following pre-digestion with chondroitinase ACII; or, (E) CS/DS GAG chains after pre-digestion with chondroitinase ABC. Controls for the three antibodies, with/without enzyme pre-treatments showed negative staining (F). 0–3 mm = centre, 3–6 mm = inner periphery, 6–9 mm = mid periphery, and 9–12 mm = outer periphery/limbus. Scale bar = 200 μm.