Table 1.
Movement of wild-type and talB mutant cells in response to cAMP gradient
| Strain | % cells moving towards cAMP source |
Locomotion speed (μm min−1) |
||||
|---|---|---|---|---|---|---|
| Strong resistance | Weak resistance | Ratio | Strong resistance | Weak resistance | Ratio | |
| Ax2 | 49.9±7.5 (10) | 55.7±7.4 (9) | 0.90 | 3.09±0.31 (100) | 6.05±0.85 (66) | 0.51 |
| HKT102 | 8.4±3.8 (11)* | 54.4±7.8 (6) | 0.16 | 1.09±0.27 (59)** | 5.68±0.47 (51) | 0.19 |
| HKT104 |
4.1±0.3 (11)* |
50.5±5.7 (8) |
0.08 |
1.39±0.20 (56)** |
7.12±0.65 (84) |
0.19 |
| Dissociated cells at the mound stage were plated between agar sheets with two conditions of mechanical resistance, and a fine glass capillary containing cAMP solution was inserted as a chemoattractant source. In the left half of the table, percentage of the cells that moved towards the cAMP source within 15 min (see Materials and methods for details of the criteria) are shown as means±s.e.m. (number of experiments). In the right half, mean translocation speeds of the cells are shown (number of cells in parentheses). Ratios of the values under strong resistance to those under weak resistance are also shown. The differences between wild-type and each of the mutant strains in the values under the strong resistance condition are statistically significant (*P<0.01, **P<0.001). | ||||||