TABLE 4.
Impact of B. subtilis 168 secretion machinery components on extracellular proteome composition
| Limiting component | Extracellular
proteins whose levels
werea:
|
Mediumb | |
|---|---|---|---|
| Reduced | Increased | ||
| Ffh depletion | Csn, Pel, PenP, TasA, WapA, WprA, XkdG, XynD, YclQ, YflE, YfnI, YlqB, YncM, YwtD, YxaK, YxkC | SodA | MG |
| SecA depletion | Csn, Pel, PenP, TasA, WapA, WprA, XkdG, XynD, YclQ, YflE, YfnI, YlqB, YncM, YwtD, YxaK, YxkC | − | MG |
| SecA inhibition by sodium azide | Csn, LipA, WapA, XynA, YolA, YvcE, YweA, YxaL | MntA, OppA, YclQ | LB |
| SecDF deletion | − | − | LB |
| SpoIIIJ/YqjG deletion | − | − | LB |
| SipS-V deletionc | YfnI | − | LB |
| SipW deletion | TasA | − | |
| Lsp deletion | AmyE, Csn, Epr, LipA, GlpQ, LytD, PenP, XepA, XkdK, XkdM, XlyA, YncM, YolA, YrpD, YwoF, YxaK, YxkC | MntA, WprA, YxeB | LB |
| Lgt deletion | − | FeuA, FhuD, LytD, MntA, MsmE, OppA, PbpC, RbsB, XepA, XlyA, YfiY, YodJ, YusA, YvcE, YwtF, YxeB | LB |
| − | OppA, OpuAC, PbpC, PstS, YcdH, YdhF, YfiY, YqiX, YusA, YrpE, YxeB | PS | |
| PrsA depletion | AbnA, AmyE, AprE, BglC, Bpr, FlgK, LytD, MntA, Mpr, NprE, OppA, Pel, PenP, Vpr, WapA, WprA, XkdK, XlyA, XynA, XynD, YbdN, Ybxl, YclQ, YhcR, YlqB, YncM, YnfF, YvcE, YwoF, YwtD, YxiA, YxkC | CitH, Ef-G, Eno, Ggt, GroEL, LipA, PdhA, PdhB, PdhD, RocF, SodA, YlqB, YvgN, YweA, YwjH | LB |
| YacD deletion | − | − | LB |
| BdbA-D deletion | − | − | LB |
| HtrA deletion | HtrA, YqxI | − | LB |
| HtrB deletion | − | HtrA, YqxI | |
| CssS deletion | HtrA, YqxI | − | |
| WprA deletion | AbnA, AprE, Csn, WprA, YncM, YxaL, YweA | BglS, Epr, FlgK, Vpr, WapA, YclQ, YwsB | LB |
| AprE, Bpr, Epr, NprB, NprE, Mpr, Vpr deletion | AbnA, AprE, BglS, Bpr, NprE, Mpr, Vpr | HtrA, WapA, YqiX, YvcE | LB |
| Total-Tat deletion | − | − | LB |
| PhoD | − | PS | |
The relative amounts of all proteins listed were reduced or increased in the media of modified B. subtilis strains. Proteins that completely disappeared from or appeared in (see Table 5) the extracellular proteome are printed in bold. Proteins were identified by 2D PAGE and subsequent MALDI-TOF mass spectrometry and/or N-terminal peptide sequencing as described by Antelmann et al. (3-6). −, no protein(s) was reduced or increased in the medium of modified B. subtilis strains.
Proteins found in the extracellular proteomes of cells grown in minimal medium with glucose (MG) (46) LB broth (rich medium) (4, 5, 6, 51, 147), or phosphate starvation medium (PS) (3, 52).
The C-terminal part of YfnI was absent only from the medium of strains lacking at least SipT and SipV (5).