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. Author manuscript; available in PMC: 2014 Oct 16.
Published in final edited form as: Hepatology. 2013 Dec 20;59(2):555–566. doi: 10.1002/hep.26712

Figure 3. c-Myc is upregulated in miR-122KO mouse livers.

Figure 3

A. Western blot analysis of c-Myc protein levels in the control and miR-122KO liver nuclear extracts using an antibody that detects both mouse and human Myc proteins. Histone H3 was used as a loading control. B. qRT-PCR analysis of endogenous c-Myc mRNA expression in Hepa and KO hepatocytes transfected with miR-122 mimic (miR122) or negative control mimic (NC) as well as in Hepa cells and wild-type (WT) hepatocytes transfected with miR-122 inhibitor (anti-miR122) or negative control inhibitor (anti-NC). C. Western blot analysis of c-Myc in KO and WT hepatocytes transfected with miR-122 mimic and inhibitor, respectively along with respective negative controls as described in 3B.