Figure 1. Cellular levels of HuR control the expression of BclxL. U2OS cells were transiently transfected with HuR-targeting or non-silencing (Ctrl) siRNA and the levels of BclxL (A), or Bcl-2 and Mcl-1 (B) were determined 72 h after transfection by western blot analysis. Signal intensities were quantified by densitometry and are shown on the right (fold). (C) U2OS cells were transiently transfected with HuR-targeting or non-silencing (Ctrl) siRNA, and 48 h later transfected with GFP- or GFP-HuR expressing plasmid as indicated. The expression levels of BclxL were determined 24 h following the plasmid transfection by western blot analysis (* denotes GFP-HuR). Signal intensities were quantified by densitometry and are shown in (D). (* p < 0.05; n = 3).