TABLE 1.

Expression from transcriptional fusions is affected by rpoD1181

Strain	rpoD allele	Locusa	β-Galactosidase activity (Miller units)b	Relative activityc
DM7358	rpoD1181	ilvCd	352 ± 5	1.5
DM7359	wte	ilvCd	235 ± 6
DM7081	rpoD1181	panCd	38.9 ± 1.3	1.6
DM7082	wt	panCd	23.6 ± 0.4
DM7114	rpoD1181	thiC	104 ± 4	1.6
DM7115	wt	thiC	63.0 ± 1.4
DM7106	rpoD1181	dksA	144 ± 2	1.3
DM7107	wt	dksA	107 ± 4
DM7108	rpoD1181	stm4065	3.7 ± 0.0	0.8
DM7109	wt	stm4065	4.5 ± 0.1
DM7201	rpoD1181	gnd	27.3 ± 0.9	1.0
DM7202	wt	gnd	26.1 ± 1.9
DM7203	rpoD1181	yigF	48.4 ± 0.2	1.0
DM7204	wt	yjgF	48.9 ± 1.9
DM7205	rpoD1181	hisD	22.6 ± 3.0	1.5
DM7206	wt	hisD	15.3 ± 1.0
DM7207	rpoD1181	ilvD	26.3 ± 0.3	1.1
DM7208	wt	ilvD	24.5 ± 1.5

^aStrains carried a MudJ fusion (7) inserted in the indicated locus.

^bCells from a full-density nutrient broth (NB) culture were inoculated into NB and grown to an optical density at 650 nm of ≈0.3. β-Galactosidase activity was determined as previously described (12, 17). A Miller unit is defined as 1 nmol of o-nitrophenyl-β-d-galactopyranoside hydrolyzed per min.

^cβ-Galactosidase activity in the rpoD1181 strain divided by activity of the wild-type strain.

^dGene products from the panBCD operon are required for synthesis of pantothenate. IlvC function is required for pantothenate synthesis when panE is absent (20).

^ewt, wild type.