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. 2014 Oct;24(10):1707–1718. doi: 10.1101/gr.174615.114

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© 2014 Haelterman et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 1. — A sequencing depth of 30× permits identification of 95% of SNVs. (A) Graph displaying the number of identified SNVs at different sequencing depths for the isogenized FRT19A X chromosome (FRT19A^iso). A 30× coverage allows identification of ∼95% of the SNVs identified at 50× coverage. (B) Percentage of the X chromosome that is covered 1×–5× (black), 5×–10× (dark gray), 10×–20× (gray), or ≥20× (light gray) at various average sequencing depths. An average sequencing depth of 30× allows reliable heterozygous SNV-calling (requiring 10 or more reads) of 95% of the X chromosome. (C) Description of SNVs identified in the X chromosome of FRT19A^iso sequenced at 48× when compared to the reference sequence (y; cn bw sp) (Adams et al. 2000).