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. Author manuscript; available in PMC: 2015 Aug 28.
Published in final edited form as: Cell. 2014 Aug 28;158(5):1187–1198. doi: 10.1016/j.cell.2014.07.034

Figure 2. The MIC Genome Is Fragmented into Hundreds of Thousands of Segments with Massive Levels of Scrambling.

Figure 2

(A) Comparison of chromosome length and number of MDS segments between 13,910 non-scrambled (blue) and 2,310 scrambled nanochromosomes (red) completely covered on single MIC contigs.

(B) A chord diagram mapping MIC ctg7180000068801 to its rearranged form, MAC Contig17454.0. Lines connect precursor (MIC) and product (MAC) MDS locations; black dotted line, an inverted MDS; all 245 MDSs (242 are scrambled) drawn in a blue to yellow MAC color gradient; IESs, gray.

(C) Length distribution of 44,191 nonscrambled and 9,841 scrambled MDSs <100 nt (excluding pointers). Inset: 150,615 nonscrambled MDSs and 16,350 scrambled MDSs excluding pointers, showing the most typical length of scrambled MDSs is <50 nt.

(D) Length distribution of 101,345 nonscrambled and 8,333 scrambled high-confidence IESs <100 nt (excluding pointers and IESs that contain other MDSs). Inset: 147,122 nonscrambled versus 9,040 scrambled IESs excluding pointers and IESs that contain other MDSs. We identified six strong cases of 0 bp MDSs (four nonscrambled [Contig7827.0 MDS3, Contig11190.0.1 MDS18, Contig13633.0 MDS3, and Contig9208.0.0 MDS18] and two scrambled [Contig6325.0.0 MDS58 and Contig1267.1 MDS7]).

(E) Length distribution of 112,125 nonscrambled IESs (excluding those that contain other MDSs) <150 nt, with one copy of the pointer included (i.e., the total length of DNA deleted).

(F) MIC genomic distance between scrambled MDSs that are consecutive in the MAC (n = 12,197); distance calculated from the pointer flanking MDS N to its paired pointer flanking MDS N+1.

See also Figure S2.