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Increased heterogeneity of OPG molecules in an mdoD mutant: DEAE-Sephacel anion-exchange column chromatography profiles of d-[U-14C]glucose-labeled OPGs from strains DF214 (mdo+) (A), NFB1967 (mdoD218::cml) (B), and NFB1567 (mdoD218::cml [pNF599]) (C). The ionic strength was increased by 0.05 M NaCl steps at fractions indicated by the arrows. Fractions (4 ml) were collected, and aliquots were counted.
