Figure 2.

Differentiated SH-SY5Y cells were treated with an amyloid β (Aβ)⁴² peptide. (A) Western blot analysis of extracted protein revealed that, in the control (lane 1), there was no Aβ⁴² deposition, but with Aβ⁴² treatment there was an accumulation of the peptide detected at 4 kDa (lane 2). (B) Quantitative polymerase chain reaction (qPCR) of cells treated with Aβ⁴² revealed a significant increase in mechanistic target of rapamycin (mTOR) expression compared to the untreated cells and a significant reduction in DEPTOR expression over 24 h. Data are the means ± standard error of the mean (SEM). ^*P<0.05, stastistically significant difference compared to the untreated control samples (n=3).