TABLE 3.
Luminescence of V. fischeri luxS mutantsa
| Strain type | Luminescence values (10−2 quanta s−1 cell−1) with addition ofb:
|
||
|---|---|---|---|
| None | C8-HSL | 3-Oxo-C6-HSL | |
| Wild type | 5.7 (0.1) | 8.2 (1.1) | 17,000 (3,900) |
| luxS | 4.2 (0.2) | 5.1 (0.4) | 22,000 (3,600) |
| ainS | BDc | 4.3 (0.2) | 5 (1) |
| ainS luxS | BD | 2.6 (0.1) | 4 (1) |
| luxO | 6.8 (0.8) | 12.2 (2.9) | 24,000 (4,000) |
| luxS luxO | 8.3 (1.1) | 17.2 (4.0) | 37,000 (1,500) |
a
Specific luminescence values are the means of cultures with an OD600 of between 1.0 and 5.0; standard errors of the means are indicated in parentheses. The results shown are from a representative experiment.
b
Cultures were grown in SWT either without additions or with the addition of either 120 nM C8-HSL or 120 nM 3-oxo-C6-HSL.
c
BD, below detection (<0.02 quanta s−1 cell−1).