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Purification of the recombinant proteins. (A) Diagram of the subclone of Gaussia luciferase (GLuc) and EC1-GLuc into the pET-52b(+) vector. (B) Coomassie brilliant blue staining and western blot analysis of purified proteins. Lanes 1 and 2, Coomassie brilliant blue staining of GLuc and EC1-GLuc, respectively. Lanes 3 and 4, western blot analysis of GLuc and EC1-GLuc, respectively.
