Figure 5.
Preparation of metastatic SKOv3 tumor-bearing mice. (A) Observation of metastatic SKOv3 tumors in mice. Three weeks after the intraperitoneal implantation of 5×106 SKOv3 cells, a laparotomy was performed to observe metastatic SKOv3 tumors in mice. The black triangular arrows indicate the metastatic SKOv3 tumors. (B) Histological observation of metastatic SKOv3 tumors using hematoxylin and eosin (H&E) staining. Scale bar, 100 μm. (C) Expression of ErbB2 in metastatic ovarian tumors derived from SKOv3 cells. Paraffin-embedded slices of tumors were subjected to immunofluorescence (IF) staining with rabbit anti-ErbB2 antibody and Hoechst 33248 for nuclear staining. Scale bars, 50 μm.