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. 2014 Sep;10(3):146–157.

Figure 4.

Figure 4

MH-S alveolar macrophage cells were simultaneously stimulated with the small molecule statin derivative LFA 878 and LMW HA (250 μg/ml) and cytokine production was measured. A, The small molecule statin derivative LFA 878 demonstrated a dose response inhibitory effect on LMW HA induced MIP 1-α expression with statistically significant inhibition noted at 20 μM (p=0.02) and maximal inhibition achieved at an 80 μM dose without evidence of cell death by Trypen blue assay (p=0.004); B) Simultaneous administration of LFA 878 (80 μM) also inhibited LMW HA induced RANTES production, although it did not reach statistical significance (p=0.08); C, There was no inhibitory effect of LFA 878 on LMW HA induced TNF-α production; D, Simultaneous administration of CD11a blocking antibodies (10 μg/ml) near the N-terminus of the I-domain inhibited LMW HA induction of MIP 1-α (Y17, p=0.022), as did an antibody directed towards talin protein that anchors LFA-1 to the cytoskeleton (H300, p=0.021). Whereas administration of M17/4 antibody specific for the carboxy-terminus of the I-domain of CD11a failed to inhibit LMW HA induction of MIP 1-α (p=0.428).