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. 2014 Oct 16;10(10):e1004703. doi: 10.1371/journal.pgen.1004703

Figure 7. natc-1 is epistatic to daf-16 in resistance to heat and zinc stress.

Figure 7

(A) Wild-type (WT), natc-1(am138), daf-2(e1370), and daf-2(e1370);natc-1(am138) animals were cultured at 15°C on NGM, shifted to 35°C as day 1 adults, and assayed for survival hourly beginning at 12 hours (N = 39–61). (B) Wild-type (WT), natc-1(am138), daf-16(mu86), and daf-16(mu86);natc-1(am138) animals were cultured at 15°C on NGM, shifted to 35°C as day 1 adults, and assayed for survival hourly. Summary statistics are presented in Table 1. (C) Embryos were cultured on NAMM with 200 µM supplemental zinc. Bars indicate the percentage of embryos that generated fertile adults. Genotypes were wild type (WT), natc-1(am138), daf-16(mu86), and daf-16(mu86);natc-1(am138) (N = 49–54). daf-16(mu86) animals were similar to wild-type animals, and natc-1(am138) caused significant zinc resistance in wild-type and daf-16(mu86) mutant animals (*, p<0.05).