Skip to main content
. 2014 Oct 16;10(10):e1004687. doi: 10.1371/journal.pgen.1004687

Figure 2. Deletion of Gpr177 in embryonic epidermis leads to ablation of Wnt/β-catenin signaling in dermis.

Figure 2

(A) Quantitative RT-PCR shows a decrease in the expression of Axin2, Dkk1, Fzd1, Lef1, TCF4 in limb skin of mutants at E16.5. **, P<0.01, compared to wild type controls. Data are represented as mean ± SD and are representatives of at least three independent experiments. (B–C, B′–C′) X-Gal staining on sections of dorsal limbs shows lack of TopGal activity in dermal mesenchyme at E13.5 and E16.5 in Gpr177K14, as compared to wild type controls (Wt). (D–G, D′–G′) In situ hybridization on the sections of embryonic limb skin shows the down-regulation of Axin2 and Lef1 in embryonic epidermal stratification of mutants at E13.5 and E14.5.