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. 2014 Oct 16;10(10):e1004708. doi: 10.1371/journal.pgen.1004708

Figure 2. Determination of domains and amino acid residues critical for mediating Tpz1-Ccq1 and Tpz1-Poz1 interaction in Y2H assays.

Figure 2

(A) Identification of minimum Tpz1-Ccq1 and Tpz1-Poz1 interaction domains by Y2H assay. A positive interaction is identified by growth on −His −Ade plates. (B) Identification of mutations that specifically disrupt Tpz1-Ccq1 interaction by Y2H assay. Indicated mutations were tested in full length Tpz1 (Tpz1-FL) or the minimum Ccq1 interaction domain construct of Tpz1 (Tpz1-[421–485]). (C) Identification of mutations that specifically disrupt Tpz1-Poz1 interaction by Y2H assay. The effect of W498R,I501R mutation was tested in full length Tpz1 (Tpz1-FL) or the minimum Poz1 interaction domain construct of Tpz1 (Tpz1-[486–508]).