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. 2014 Oct 16;10(10):e1004701. doi: 10.1371/journal.pgen.1004701

Figure 1. Ethylene responses in etiolated mhz4 seedlings.

Figure 1

Rice seedlings were grown in dark for 3 d in the presence of various concentrations of ethylene. (A) Ethylene-response phenotypes of WT (Nipponbare) and mhz4 seedlings grown in air or 10 ppm ethylene. Bar = 10 mm. (B) Ethylene dose-response curves for root length (top) and relative root length (bottom) in WT and mhz4 seedlings. Each point is average of 20 to 30 seedlings and bars indicate SD. (C) Ethylene dose-response curves for coleoptile length (top) and relative coleoptile length (bottom) of WT and mhz4 seedlings. Others are as in (B). (D) Expression of ethylene-inducible genes in both shoot and root of WT and mhz4 seedlings. Dark-grown 2 d-old seedlings were treated with or without 10 ppm ethylene for 8 h and the RNA was isolated for quantitative PCR. Data are the mean ± SD of four replicates. (E) Expression of ethylene-inducible genes in root (left) or shoot (right) of WT and mhz4 seedlings. Others are as in (D).