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. 2014 Oct 16;10(10):e1004701. doi: 10.1371/journal.pgen.1004701

Figure 6. MHZ4 overexpression confers enhanced and reduced ethylene responses in roots and coleoptiles, respectively.

Figure 6

(A) Ethylene response phenotypes in WT and MHZ4-overexpressing (MHZ4-OX) lines. Rice seedlings were grown in dark for 2.5 d in the presence or absence of 1 ppm ethylene. Bar = 10 mm. (B) Ethylene dose-response curves for root length (top) and relative root length (bottom) in WT and MHZ4-OX lines. (C) Ethylene dose-response curves for coleoptile length (top) and relative coleoptile length (bottom) in WT and MHZ4-OX lines. Others are as in (B). (D) Expressions of ethylene-inducible genes in roots (top) and shoots (bottom) of WT and MHZ4-OX lines. Dark-grown 2 d-old seedlings were treated with or without 10 ppm of ethylene for 8 h and the RNA was isolated for quantitative RT-PCR. Data are the mean ± SD of four replicates. (E) OsEIN2 gene expression in shoots of WT and MHZ4-OX lines detected by semi-quantitative PCR. Rice seedlings were grown in dark for 3 days and RNA was isolated from shoots. Actin1 was used as an internal control.