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. 2004 Jun;186(12):4000–4013. doi: 10.1128/JB.186.12.4000-4013.2004

FIG. 5.

FIG. 5.

Immunolocalization patterns of β-galactosidase produced from the sspE-lacZ fusion. The strains of B. subtilis were grown in DSM, and samples were taken at the indicated times after the onset of sporulation, stained with DAPI, and processed for immunofluorescence microscopy as described in Materials and Methods. Typical localization patterns of β-galactosidase (β-gal.) produced from the sspE-lacZ fusion for strains AH3786 (ΔspoIIIG sspE-lacZ ΔamyE::spoIIIG) (wild-type spoIIIG in a wild-type background) (a to c), AH3787 (ΔspoIIIG sspE-lacZ ΔamyE::spoIIIGE156K) (d to f), AH3790 (ΔspoIIIG spoIIIA::Tn917 sspE-lacZ ΔamyE::spoIIIG) (wt) (g to i), and AH3791 (ΔspoIIIG spoIIIA::Tn917 sspE-lacZ ΔamyE::spoIIIGE156K) (j to o) at the indicated times after the onset of sporulation are shown. The complete relevant genotypes of the strains are given in Table 1. The samples were taken 6 or 10 h after the onset of sporulation (T6 and T10, respectively). Arrowheads point to specimens showing sspE-lacZ expression. The specimen labeled with an asterisk in panel f, as well as the specimens in panels l and o, show whole-cell fluorescence (see text). Bars, 2 μm.