FIG. 8.

Immunoblot analysis of σ^G accumulation produced in the mother cell during sporulation. σ^G accumulation in B. subtilis strains AH2460 (ΔspoIIIG sspE-lacZ P_spoIID-spoIIIG) (wild-type spoIIIG in a wild-type background) (A), AH2461 (ΔspoIIIG sspE-lacZ P_spoIID-spoIIIGE156K) (B), AH2463 (ΔspoIIIG sspE-lacZ lonA::cat P_spoIID-spoIIIG) (wt) (C), and AH2465 (ΔspoIIIG sspE-lacZ lonA::cat P_spoIID-spoIIIGE156K) (D) during sporulation in DSM was examined by immunoblot analysis. Samples from sporulating cultures were collected 1 h after the onset of sporulation in DSM and at hourly intervals thereafter, as indicated by the numbers above the lanes. Proteins (30 μg) in each sample were subjected to immunoblot analysis using an anti-σ^G rabbit polyclonal antibody (see Materials and Methods). Lanes Δ IIIG contain 30-μg portions of an extract prepared from a culture of a spoIIIG deletion mutant (AH3795 [Table 1]) 4 h after the onset of sporulation. The position of σ^G is indicated by an arrowhead. Other bands represent nonspecific cross-reactive material. One band, which appears close to σ^G in the spoIIIG deletion mutant, is marked with an asterisk for reference.