TABLE 4.
Strain | Genotype | Time (h)a | No. countedb | Sporulating cells/%c | Stage III cells/%d |
---|---|---|---|---|---|
AH3786 | spoIIIG wte | 2 | 279 | 91/33 | 0 |
4 | 143 | 101/71 | 60/42 | ||
6 | 132 | 90/68 | 64/48 | ||
8 | 131 | 100/76 | 86/66 | ||
10 | 115 | 93/81 | 83/72 | ||
AH3787 | spoIIIGE156K | 2 | 487 | 153/31 | 0 |
4 | 178 | 121/68 | 55/31 | ||
6 | 357 | 221/62 | 53/51 | ||
8 | 158 | 133/84 | 117/74 | ||
10 | 172 | 129/75 | 114/66 | ||
AH3790 | spoIIIA spoIIIG wt | 2 | 157 | 70/45 | 0 |
4 | 172 | 121/70 | 54/31 | ||
6 | 167 | 119/71 | 98/59 | ||
8 | 102 | 70/69 | 53/52 | ||
10 | 152 | 67/44 | 48/32 | ||
AH3791 | spoIIIA spoIIIGE156K | 2 | 219 | 77/35 | 0 |
4 | 260 | 110/42 | 50/20 | ||
6 | 135 | 87/64 | 52/39 | ||
8 | 111 | 76/68 | 60/54 | ||
10 | 153 | 70/46 | 54/35 |
Hours after the onset of sporulation.
The total number of cells counted is indicated.
The number of sporulating cells, estimated on the basis of the pattern of staining by FM4-64 and MTG (see text), is shown before the slash, and the percentage of sporulating cells is shown after the slash.
The number of cells in stage III or above is shown before the slash, and the percentage of cells in stage III or above is shown after the slash.
spoIIIG wt, wild-type spoIIIG.