TABLE 5.
Patterns of β-galactosidase localization
| Strain | Genotype | Time (h)a | No. countedb | Sporulating cells/%c | Localization of β-galactosidased
|
||
|---|---|---|---|---|---|---|---|
| Prespore | MC | Whole cell | |||||
| AH3786 | spoIIIG wt | 6 | 411 | 250/61 | 187 | 0 | 0 |
| 10 | 360 | 358/99 | 9 | 0 | 0 | ||
| AH3787 | spoIIIGE156K | 6 | 446 | 305/68 | 222 | 0 | 9 |
| 10 | 413 | 397/96 | 10 | 0 | 3 | ||
| AH3790 | spoIIIA spoIIIG wt | 6 | 193 | 151/78 | 0 | 0 | 0 |
| 10 | 178 | 107/60 | 1 | 0 | 0 | ||
| AH3791 | spoIIIA spoIIIGE156K | 6 | 431 | 309/75 | 0 | 2 | 12 |
| 10 | 587 | 187/32 | 0 | 1 | 135 | ||
a
Hours after the onset of sporulation.
b
The total number of cells counted is indicated.
c
The number of sporulating cells, estimated on the basis of the pattern of DAPI staining (see text), is shown before the slash, and the percentage of sporulation is shown after the slash.
d
The number of cells or free spores in prespores, mother cells (MC), and whole cells (cells with no signs of prespore).