Figure 3. a-Pheromone (MFa1) and the α-pheromone response pathway (Ste2-MAPK-Cph1) of a cells are required for a white cells to induce mating projection formation in α opaque cells (GH1349).

Strains used: GH1013, GH1609, GH1610, GH1613, GH1614, and GH1615. (A) Cellular images of single strain cultures. (B) Cellular images of mixed cultures. Opaque α cells (GH1349) were mixed with white a cells of different mutants as indicated. This experiment was performed as described in Figure 1B. Percentages of opaque α cells (GH1349) with mating projections are shown below the images. W, white; O, opaque; P, mating projection. Scale bar, 10 µm.