Figure 6.

Conceptual presentation of T4 lysozyme multidimensional conformational dynamics. T4 lysozyme exhibits multiple conformational intermediate states during open–close hinge-bending motions, involving multiple domain orientation coordinates and FRET coordinate. FRET-coordinate projections of multiple T4 lysozyme conformations associated with different domain orientation coordinates are presented. Along domain orientation coordinates, different enzyme conformations can have the same D–A distance, which can be undetectable and hidden in a conventional single-molecule FRET spectroscopic measurement.