Figure 5. Hypoxia induces mesenchymal markers.

A549 cells were cultured under normoxic or hypoxic conditions (4% O₂) for 24 or 48 hours. Vimentin (A) and PAI1 (B) mRNA levels were measured by qPCR and are expressed as mean+SD, * indicates p<0.05 compared to control. C57BL/6J mice were exposed to normoxia (n = 8) or hypoxia (n = 7) for 21 days and additionally a pair-fed group (n = 8) was used. Lung fibronectin (C) and PAI1 (D) mRNA levels were determined by qPCR. Data are expressed as mean, * indicates p<0.05.