Table 1. Primer sequences.
Gene | Forward primer 5′ to 3′ | Reverse primer 5′ to 3′ |
Human | ||
E-cadherin | TCATGAGTGTCCCCCGGTAT | GTCAGTATCAGCCGCTTCAGAT |
Keratin 7 | GGGACTGCAGCTCTGTCAAC | CTGCCTACATGAGCAAGGTG |
Keratin 18 | CGGGCATTGTCCACAGTATT | GGGAGCACTTGGAGAAGAAG |
α Smooth muscle actin | GAGAAGAGTTACGAGTTGCCTGA | TGTTAGCATAGAGGTCCTTCCTG |
Plasminogen activator inhibitor 1 | GAAAGTGAAGATCGAGGTGAACGAGA | CATGCGGGCTGAGACTATGACA |
Vimentin | ATTCCACTTTGCGTTCAAGG | CTTCAGAGAGAGGAAGCCGA |
Transforming growth factor-β | TGAACCGGCCTTTCCTGCTTCTCATG | GCGGAAGTCAATGTACAGCTGCCGC |
SMAD7 | TACCGTGCAGATCAGCTTTG | TTTGCATGAAAAGCAGCAC |
Carbonic anhydrase 9 | CATCCTAGCCCTGGTTTTTGG | GCTCACACCCCCTTTGGTT |
Ribosomal Protein L13a | CCTGGAGGAGAAGAGGAAAGAGA | TTGAGGACCTCTGTGTATTTGTCAA |
Mouse | ||
Plasminogen activator inhibitor 1 | AGTCTTTCCGACCAAGAGCA | GACAAAGGCTGTGTGGAGGAAG |
Fibronectin | GTGTAGCACAACTTCCAATTACGAA | GGAATTTCCGCCTCGAGTCT |
Ribosomal protein L13a | CACTCTGGAGGAGAAACGGAAGG | GCAGGCATGAGGCAAACAGTC |
Carbonic anhydrase 9 | CAGGAGGCCTGGCAGTTTT | TTCTTCCAAATGGGACAGCAA |