Table 1. P. aeruginosa strains and plasmid used in this study.

	Description	Source
Strains
PAO1	Prototroph; human isolate	(Holloway et al., 1979)
PA103	Human isolate	(Liu, 1966)
PAK	Prototroph; human isolate	(Strom and Lory, 1986)
PA14	UCBPP-PA14; burn wound isolate	(Rahme et al., 1995)
PAOΔpilA	pilA inactivated by allelic displacement; tagged with eGFP, in a mini-Tn7 construct; Gmr	(Klausen et al., 2003)
PA14ΔfimV*	PA14 with TnMAR2xT7 insertion in fimV; Gmr	(Liberati et al., 2006)
PA14ΔpilG	PA14 with TnMAR2xT7 insertion in pi/G; Gmr	(Liberati et al., 2006)
PA14ΔpilH	PA14 with TnMAR2xT7 insertion in pi/H; Gmr	(Liberati et al., 2006)
PA14ΔpilI	PA14 with TnMAR2xT7 insertion in pi/I; Gmr	(Liberati et al., 2006)
PA14ΔpilJ	PA14 with TnMAR2xT7 insertion in pi/J; Gmr	(Liberati et al., 2006)
PA14ΔpilK	PA14 with TnMAR2xT7 insertion in pi/K; Gmr	(Liberati et al., 2006)
PA14ΔchpA	PA14 with TnMAR2xT7 insertion in chpA; Gmr	(Liberati et al., 2006)
PA14ΔpilR	PA14 with TnMAR2xT7 insertion in pi/R; Gmr	(Liberati et al., 2006)
PA14ΔrpoN	PA14 with TnMAR2xT7 insertion in rpoN; Gmr	(Liberati et al., 2006)
PW1762†	chpC-F07::IS/acZ/hah at bp 79 or 507 in the ORF; Tcr	(Jacobs et al., 2003)
Plasmids
pMRP9-1	pUCP18 carrying a gene encoding enhanced green fluorescent protein (GFP); Cbr	(Davies et al., 1998)
pUCP19	E. coli-P. aeruginosa shuttle vector; Cbr	(Schweizer, 1991)
pKF906	pUCP19 carrying intact vfr; Cbr	(West et al., 1994)
pMP220::PpvdS	pMP220 carrying a pvdS promoter transcriptional lacZ fusion, Tcr	(Ambrosi et al., 2002)

^*PA14 mutants were purchased from the PA14 Transposon Insertion Mutant Library of non-redundant Pseudomonas aeruginosa transposon insertion mutants (http://ausubellab.mgh.harvard.edu/cgi-bin/pa14/home.cgi [accessed 22 January 2014])

^†PAO1 transposon mutant purchased from the University of Washington Genome Center (http://www.gs.washington.edu/labs/manoil/libraryindex.htm [accessed 22 January 2014])

^rresistant; Cb, carbenicillin; Gm, gentamicin; Tc, tetracycline