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. 2014 Oct 16;9(10):e109737. doi: 10.1371/journal.pone.0109737

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© 2014 Uchiyama et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Brains from terminally ill, 22L-inoculated Prnp^0/+, Tg(MHM2Δ23-88)/Prnp^0/+, and Tg(MHM2Δ23-88)/Prnp^0/0 mice, and from asymptomatic, 22L-inoculated Tg(MHM2Δ23-88)/Prnp^0/0 mice (sacrificed at 337 dpi) were subjected to Western blotting using M20 and 3F4 anti-PrP antibodies after treatment with or without PK. M20 antibodies detect wild-type and mutant PrPs. 3F4 antibody recognizes only the mutant PrP. The PK-resistant MHM2Δ23-88, or MHM2^ScΔ23-88, is detectable in terminally ill, 22L-inoculated Tg(MHM2Δ23-88)/Prnp^0/+ and Tg(MHM2Δ23-88)/Prnp^0/0 mice, but not in asymptomatic, 22L-inoculated Tg(MHM2Δ23-88)/Prnp^0/0 mice. β-actin is an internal control.