Table 2. Titration of the brain homogenates of RML- or 22L-infected terminally ill C57BL/6 wild-type mice.
| Dilution of brain homogenates | Incubation timesb (No. of diseased mice/No. of inoculated mice) | |
| RML | 22L | |
| 10−1 | 135±1 (6/6) | 133±2 (6/6) |
| 10−4 | 220±14 (4/5) | 228±15 (5/5) |
| 10−5 | 251±49 (3/6) | 262±31 (5/6) |
| 10−6 | >365 (0/6) | 308 (1/6) |
| Infectious titers (ID50/gram of tissue)a | 107.7 | 108.2 |
a
ID50/gram of the tissue was calculated by the method of Reed and Muench [13].
b
Incubation times are indicated as average ± standard deviation (days).