Figure 1. Melanoma and glioma cell lines are permissive to recombinant myxoma virus infection.
Cell lines (A) RK-13, (B) GL261, (C) B16-F10, (D) B16.SIY, were infected with either vMyx-tdTr or vMyx-IL15Rα-tdTr at a multiplicity of infection (MOI) of 0.1 to obtain multi-step growth curves. At 0, 12, 24, 48, 72 or 96 h post-infection (p.i.), cells were harvested and lysed, and the viral titer was determined by titration on RK-13 cells. Error bars represent SEM from 3 replicates for each cell line. There was a significant effect of time for each of the cell lines (p<0.001).