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. 2014 Oct 16;9(10):e109801. doi: 10.1371/journal.pone.0109801

Figure 6. Analysis of subsets of T cells infiltrating subcutaneous B16-F10 tumors 3 days after intratumoral virus treatment in C57BL/6 mice.

Figure 6

C57BL/6 mice (n = 3 per group) were implanted with unilateral subcutaneous B16-F10 tumor cells. The first dose of the virus (2.6×107 PFU i.t.) was given on day 7 (when tumors reached approximately 100 mm3) and the second dose was given on day 10. Treatment groups are: 1. vMyx-IL15Rα-tdTr, 2. vMyx-tdTr, 3. PBS. Mice were euthanized 3 days after the final virus treatment and tumor sections were analyzed for presence of T cells by immunostaining for CD4 and CD8 markers. Representative tumor sections are shown. (A) Staining for CD8+ T cells in tumors. Green – CD8-positive cells, Blue – DAPI. Scale bar  = 50 micrometers. (B) Staining for CD4+ T cells in tumors. Red – CD4-positive cells, Blue – DAPI. Scale bar  = 50 micrometers.