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. 2014 Oct 16;9(10):e109418. doi: 10.1371/journal.pone.0109418

Figure 2. Cyr61 expression in Müller cells was observed at mRNA and protein level.

Figure 2

A) Cells were harvested for total mRNA. Cyr61 mRNA levels was assessed by RT-qPCR at 10 mM glucose for 0–24 h. There was a time-dependent increase in expression of Cyr mRNA. B) To further understand the interaction of Cyr61 and high glucose, we examined the Cyr61 expression in Müller cells exposed to different glucose concentrations (0–20 mM) for 8 h at mRNA level. There was a does-dependent increase in expression of Cyr mRNA C) Cyr61 protein expression of Müller cells under different glucose concentrations (0–20 mM) was assessed by Western blot analysis (left) and then quantified (right). There was a does-dependent increase in expression of Cyr61 protein. Cyr61 expression was normalized by GAPDH expression. Results shown are the mean ± SD of three independent experiments. * P<0.05; ** P<0.01.