Table 2. ANOVA and mean comparison by Tukey's method of ELISA OD values of positive, SPF and sera from vaccinated chicks.

Mean Comparison
Test group	Number*	Mean	SE Mean	Grouping
tM2e+Challenged	9	1.46	0.186	A
M2e+Challenged	9	0.62	0.173	B
tM2e+Vaccinated	9	0.06	0.029	C
M2e+Vaccinated	9	−0.13	0.065	C
tM2e+SPF	9	0	0.002	C
M2e+SPF	9	0	0.001	C

* From each positive, vaccinated and SPF groups, three samples were tested in triplicate in both tM2e-MBP and M2e-MBP ELISAs.