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. 2014 Oct 16;9(10):e109880. doi: 10.1371/journal.pone.0109880

Figure 3. Hatching rate and morphological alterations of zebrafish embryos.

Figure 3

DFO and L1-treated embryos hatched successfully, while ICL-670-treated embryos hatched less successfully than control due to high mortality (A). Bars missing at 1 mM are due to the death of embryos prior to hatching. § denoted the concentration of L1 that was excluded due to high DMSO content. There were no significant defects observed in DFO treated embryos. However, the percentage of embryos demonstrating behavioral and morphological defects increased in a time and concentration dependent manner in L1 and ICL-670-treated embryos (B). The time of onset of morphological and behavioral alterations is outlined in the figure as a separate panel. Swimming behavior was affected and bent bodies ( Figure 5 ) were observed at concentrations above 0.25 mM for ICL 670-treated embryos and at 0.5 mM for L1 treated embryos.