Table 2. The forward and reverse primers used for real-time qPCR.
Gene | Primer | Accession No. Gen Bank | Amplicon size, bp |
Hamp 1 | F: 5′-CCGAGCAGAAGACAAGTAGAT-3′R: 5′-GCAGCCAGAAACACGTTAGA-3′ | NM_205583.1 | 104 |
DMT-1 | F: 5′-ACCGCAGCAATAAGAAGGAG-3′R: 5′-TTGGTTTTCCCGTAGAAGGC-3′ | NM_001040370.1 | 136 |
Ferroportin | F: 5′-ATTTACTTTGCCCGAGCCTT-3′R: 5′-CAGCGAGGTTTCTTTGATGC-3′ | NM_131629.1 | 104 |
β-Actin | F: 5′-TTCAAACGAACGACCAACCT-3′R: 5′-TTCCGCATCCTGAGTCAATG-3′ | NM_131031.1 | 93 |