TABLE 1.
Plasmids used in this study
| Plasmida | Encoded protein(s) |
|---|---|
| pHM462 | Cys-less SecY-His6-Myc |
| pHM464 | SecY(A136C)-His6-Myc |
| pHM465 | SecY(Q131C)-His6-Myc |
| pHM466 | SecY(I129C)-His6-Myc |
| pHM467 | SecY(Y122C)-His6-Myc |
| pHM468 | SecY(S132C)-His6-Myc |
| pHM469 | SecY(A128C)-His6-Myc |
| pHM470 | SecY(Q118C)-His6-Myc |
| pHM471 | SecY(T120C)-His6-Myc |
| pHM472 | SecY(T137C)-His6-Myc |
| pHM473 | SecY(P140C)-His6-Myc |
| pHM474 | SecY(Y119C)-His6-Myc |
| pHM475 | SecY(G123C)-His6-Myc |
| pHM476 | SecY(T124C)-His6-Myc |
| pHM477 | SecY(L125C)-His6-Myc |
| pHM478 | SecY(L127C)-His6-Myc |
| pHM479 | SecY(F130C)-His6-Myc |
| pHM480 | SecY(I135C)-His6-Myc |
| pHM481 | SecY(V126C)-His6-Myc |
| pHM482 | SecY(I133C)-His6-Myc |
| pHM483 | SecY(G134C)-His6-Myc |
| pHM484 | SecY(G138C)-His6-Myc |
| pHM485 | SecY(L139C)-His6-Myc |
| pHM486 | SecY(N141C)-His6-Myc |
| pHM487 | SecY(R121C)-His6-Myc |
| pHM488 | SecY(G123C I135C)-His6-Myc |
| pNA3 | SecY-His6-Myc |
| pNA61 | SecY(Y157C)-His6-Myc |
| pNA62 | SecY(F158C)-His6-Myc |
| pNA63 | SecY(T159C)-His6-Myc |
| pNA64 | SecY(A160C)-His6-Myc |
| pNA65 | SecY(V161C)-His6-Myc |
| pNA66 | SecY(V162C)-His6-Myc |
| pNA67 | SecY(S163C)-His6-Myc |
| pNA68 | SecY(L164C)-His6-Myc |
| pNA69 | SecY(V165C)-His6-Myc |
| pNA70 | SecY(T166C)-His6-Myc |
| pNA71 | SecY(G167C)-His6-Myc |
| pNA72 | SecY(T168C)-His6-Myc |
| pNA73 | SecY(M169C)-His6-Myc |
| pNA74 | SecY(F170C)-His6-Myc |
| pNA75 | SecY(L171C)-His6-Myc |
| pNA76 | SecY(M172C)-His6-Myc |
| pNA77 | SecY(W173C)-His6-Myc |
| pNA78 | SecY(L174C)-His6-Myc |
| pNA79 | SecY(G175C)-His6-Myc |
| pNA80 | SecY(E176C)-His6-Myc |
| pSA61 | SecE SecG(L10C) |
| pSA62 | SecE SecG(I11C) |
| pSA63 | SecE SecG(V12C) |
| pSA64 | SecE SecG(A13C) |
| pSA65 | SecE SecG(I14C) |
| pSA66 | SecE SecG(G15C) |
| pSA67 | SecE SecG(L16C) |
| pSA68 | SecE SecG(V17C) |
| pSA69 | SecE SecG(T61C) |
| pSA70 | SecE SecG(L62C) |
| pSA71 | SecE SecG(F63C) |
| pSA72 | SecE SecG(F64C) |
| pSA73 | SecE SecG(I65C) |
| pSA74 | SecE SecG(I66C) |
| pSA75 | SecE SecG(S67C) |
| pSA76 | SecE SecG(L68C) |
| pSTD343 | LacIq |
| pTYE100 | SecE SecG |
| pTWV228 | Vector |
| pSTV28 | Vector |
a
pHM462 to pHM488 and pNA3 to pNA80 are pBR-based plasmids. pSA61 to pSA76 are pACYC184-based plasmids. pHM462 to pHM488, pNA61 to pNA80, and pSA61 to pSA76 were constructed in this study. pNA3 (12) and pTYE100 (13) were described previously.