TABLE 1.
Microarray analysis of genes controlled by ArgR and l-arginine under aerobic growth conditions in P. aeruginosa strains PAO1 and PAO501 (argR::GmΩ)
| Gene no.a | Gene name | Maximum changeb | Protein description or function |
|---|---|---|---|
| PA0328 | 3.7 | Unknown | |
| PA0888 | aotJ | 6.0 | Arginine:ornithine ABC transporter |
| PA0889 | aotQ | 8.7 | Arginine:ornithine ABC transporter |
| PA0890 | aotM | 7.4 | Arginine:ornithine ABC transporter |
| PA0891 | 4.9 | Unknown | |
| PA0892 | aotP | 3.2 | Arginine:ornithine ABC transporter |
| PA0893 | argR | 6.2 | Arginine-responsive transcriptional regulator |
| PA0895 | aruC | 26.1 | AST pathway for arginine utilization |
| PA0896 | aruF | 55.0 | AST pathway for arginine utilization |
| PA0897 | aruG | 25.7 | AST pathway for arginine utilization |
| PA0898 | aruD | 30.3 | AST pathway for arginine utilization |
| PA0899 | aruB | 29.5 | AST pathway for arginine utilization |
| PA0900 | 4.8 | Unknown | |
| PA0901 | aruE | 7.7 | AST pathway for arginine utilization |
| PA1818 | adcA | 19.8 | Putative catabolic ADC |
| PA1819 | adcB | 6.2 | Putative arginine:agmatine antiporter |
| PA1971 | braZ | 18.2 | Permease for branched-chain amino acids |
| PA2042 | 4.3 | Sodium:serine symporter; ygjU | |
| PA3068 | gdhB | 19.9 | Catabolic NAD-dependent glutamate dehydrogenase |
| PA3934 | 8.9 | Putative short oligopeptide transporter | |
| PA5152 | 5.7 | ABC transporter | |
| PA5153 | 3.2 | ABC transporter | |
| PA5154 | 5.0 | ABC transporter | |
| PA5155 | 4.1 | ABC transporter | |
| PA5170 | arcD | 7.5 | Arginine:ornithine antiporter |
| PA5171 | arcA | 5.0 | ADI pathway for arginine utilization |
| PA5172 | arcB | 3.8 | ADI pathway for arginine utilization |
| PA5173 | arcC | 3.7 | ADI pathway for arginine utilization |
| PA3525 | argG | −6.7 | Arginine biosynthesis; argininosuccinase |
| PA3537 | argF | −12.2 | Arginine biosynthesis; omithine carbamoyltransferase |
| PA3538 | −3.9 | Transport of small molecules | |
| PA4588 | gdhA | −3.5 | Anabolic NADP-dependent glutamate dehydrogenase |
| PA4756 | carB | −3.3 | Carbamoylphosphate synthetase, heavy subunit |
| PA4757 | −2.1 | Unknown | |
| PA4758 | carA | −3.2 | Carbamoylphosphate synthetase, light subunit |
| PA5035 | gltD | −2.2 | Glutamate synthase, light subunit |
| PA5036 | gltB | −4.1 | Glutamate synthase, heavy subunit |
a
Gene numbers are from the Pseudomonas Genome Project (www.pseudomona.scom). Boldface type indicates genes or gene products previously reported to be controlled by arginine and ArgR.
b
Maximum changes (fold) in gene expression are indicated by the ratio of PAO1 (wild type) to PAO501 (argR) grown in glutamate minimal medium in the presence of arginine for arginine induction genes and the ratio of PAO501 to PAO1 with a minus sign for arginine repression genes.