Table 1. Candidate genes and SNP markers at the major sex determining region of turbot (through comparative mapping against the stickleback LGVIII chromosome.
| Gene | Stickleback Genome Position, bp | Accession No. | External Primers | Internal Primer | Amplicon Size, pb | Marker Position |
|---|---|---|---|---|---|---|
| dnaj19 | 6068380 - 6070562 | KJ434933 | F: GCCGTGAAGCAGATGGAG | F: CCACCGGTGATAGTTGTGG | 392 | Third intron |
| R: GGGAAACAATCAATGGATCA | ||||||
| sox2 | 6157588 - 6158556 | KJ434936 | F: AGGAAAGTCTCCTGGAAGGAA | R: GTCCCTTTTTCTTTCCAATGTG | 662 | 3′ UTR |
| R: CAGATGAAAAGTGGGAGACG | ||||||
| atp11b | 6308502 - 6339588 | KJ434932 | F: AGACTCATTTCTGGACGTGGA | F: GTGGACATGCAGTAGAATAACTGG | 370 | 30th intron |
| R: CACCACGTCGGGAAAGAG | ||||||
| dlg1 | 6549844 - 6552127 | KJ434935 | F: CAGGAAGAGACTCTGCTCACC | R: TCTTTAAATCCACACTGGGTGATAC | 370 | Third intron |
| R: GAATGGAAGTTTGACGTTGGA | ||||||
| fkbp2 | 6565695 - 6568855 | KJ434934 | F: CGAGAAGAGGAAGCTCGTCA | F: TTCCCCAAGTTCTGACTTTGAG | 334 | Intron |
| R: TTGGATGGAGCAAATCTACTGA | ||||||
| ncbp2 | 6569593 - 6572048 | KJ434931 | F: GCGTTGATCAGCGACTCCTA | R: CCGTGTTTGCTAACGGCT | 352 | First intron |
| R: GCAATGAGTCCGAACACAAA |
SNP, single-nucleotide polymorphism; LG, linkage group; UTR, untranslated region.