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| Method | Embryos | G0 Adult | HDR Rate, % (n) | ||||
|---|---|---|---|---|---|---|---|
| Total | Survival Rate, %* | Fertile | Fertile Rate, %** | HDR-Yielding G0/Fertile G0 | HDR-Positive G1/Total G1 | ||
| Cas9 | 50 | 7 | 14 | 3 | 42.9 | 66.7 (2/3) | 30.5 (111/364) |
| Cas9D10A | 54 | 8 | 14.8 | 8 | 100 | 12.5 (1/8) | 0.29 (3/1021) |
The survival rate is calculated as the percentage of total G0 adults divided by embryos injected.
The fertile rate is calculated as the percentage of fertile G0 flies divided by total G0 adults.
