FIG. 3.
The frequency of Lp02 transformation is dependent on DNA source and quantity. (A) Transformation frequency as a function of plasmid DNA quantity. Reporter strain JV1103 was grown on solid media at 30°C in the presence of various quantities of pJB957 (Kanr) plasmid DNA. After 48 h, bacteria were plated on selective media versus nonselective media in order to determine the number of kanamycin-resistant transformants in the total cell population. (B) Transformation frequency as a function of chromosomal DNA quantity. Transformation was assayed as in panel A using JV1160 (Kanr) chromosomal DNA instead of plasmid DNA. The limit of detection for transformation frequency was 10−9; error bars indicate standard deviations.