TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant property(ies) | Reference or source |
|---|---|---|
| L. pneumophila | ||
| Philadelphia-1 | Clinical isolate, serogroup 1 | CDCa (Atlanta, Ga.) |
| AA100 | Clinical isolate, serogroup 1 | 1 |
| JR32 | Philadelphia-1 Smr | 41 |
| Lp01 | Philadelphia-1 rpsL hsdR | 3 |
| Lp02 | Lp01 thyA | 3 |
| JV1103 | Lp02 Cmr | This study |
| JV1160 | Lp02 Kanr | This study |
| JV1727 | AA100 comR::mini-Tn10kan | This study |
| JV1729 | AA100 proQ::mini-Tn10kan | This study |
| JV1763 | JV1727 + vector | This study |
| JV1766 | JV1727 + Lp02 comR | This study |
| JV1767 | JV1727 + AA100 comR | This study |
| JV1768 | JV1729 + vector | This study |
| JV1770 | JV1729 + Lp02 proQ | This study |
| JV1769 | JV1729 + AA100 proQ | This study |
| E. coli | ||
| DH5α::λpir | DH5α(λpir) tet::Mu | 23 |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB laclqZΔM15 Tn10 (Tetr)] | Stratagene (La Jolla, Calif.) |
| Plasmids | ||
| pJK211-2 | Contains mini-Tn10 (Kanr/R6K ori) | James Kirby |
| pBluescript II KS+ | ColE1 cloning vector | Stratagene |
| pWSK29 | pSC101 cloning vector | 51 |
| pJB955 | pBluescript II KS+, 3.2-kb Lp02 insert | This study |
| pJB957 | pJB955, Kanr in center of insert | This study |
| pJB964 | pJB955, Cmr in center of insert | This study |
| pJB1389 | pJB955, Gentr in center of insert | This study |
| pJB1199 | pBluescript II KS+, 1-kb Lp02 insert, Kanr in center of insert | This study |
| pJB1517 | pWSK29, 500-bp Lp02 insert, Kanr in center of insert | This study |
| pJB1519 | pWSK29, 160-bp Lp02 insert, Kanr in center of insert | This study |
| pJB908 | RSF1010 cloning vector | 43 |
| pJB1301 | pJB908 derivative | This study |
| p34S-Gm | Cloning vector with Gentr cassette | 12 |
| pJB1653 | RSF1010 cloning vector, Gentr | This study |
| pJB1659 | pJB1653, proQ+ (from strain AA100) | This study |
| pJB1661 | pJB1653, proQ+ (from strain Lp02) | This study |
| pJB1663 | pJB1653, comR+ (from strain Lp02) | This study |
| pJB1665 | pJB1653, comR+ (from strain AA100) | This study |
a
CDC, Centers for Disease Control and Prevention.