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. 2014 Oct 16;9(10):e110683. doi: 10.1371/journal.pone.0110683

Figure 5. Maternal Effect Gene ZAR1 mRNA Abundance is Reduced in High A4 Compared with Low A4 Cows.

Figure 5

Quantitative RT-PCR results for ZAR1 (A), NLRP5 (B), DPPA3 (C), and DNMT1 (D); in cumulus oocyte complexes of High A4 (black bars, n≥3) and Low A4 (white bars, n≥5) cows. The geometric mean of GAPDH and RPL-15 was used as an endogenous control. Graphs represent a fold change in mRNA abundance with Low A4 set as control (1). Data for NLRP5 and DPPA3 were log transformed to meet normal distribution assumptions. The mean ± SEM normalized values are presented. *, P≤0.05.