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. 2014 Oct 16;9(10):e110610. doi: 10.1371/journal.pone.0110610

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© 2014 Picco et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. List of the top up-regulated genes (cut-off 1.5) in U87MG cells silenced for caspase-2 expression. B. Cellular lysates from U87MG cells transfected with the indicated siRNAs against caspase-2 or the relative control siRNAs were generated and after immunoblot were probed with an anti-caspase-2 antibody. P62, nucleoporin was used as loading control. C. mRNA expression levels of CYP1B1, CYP51A1 and LIPA were measured using qRT-PCR in U87MG cells transfected with the indicated siRNAs against caspase-2 or the relative control siRNAs. Data are presented as mean ± SD; n = 3. D. mRNA expression levels of CYP1B1, CYP51A1 and LIPA were measured using qRT-PCR in IMR90-E1A cells transfected with siRNA1 against caspase-2 or the control siRNA. Data are presented as mean ± SD; n = 3.