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. 2014 Oct 16;10(10):e1004388. doi: 10.1371/journal.ppat.1004388

Figure 2. The tombusvirus p33 replication protein interacts with the yeast Osh6p protein in the ER.

Figure 2

(A–B) Left panels: BiFC analysis of interaction between Osh6p and p33 in yeast cells. Confocal laser microscopy images also show the peroxisomal localization of Pex13-CFP or ER-localized Pho86-CFP. The merged images demonstrate the interaction between p33 and Osh6p (detected via BiFC) and their partial co-localization with Pex13-CFP and Pho86-CFP. DIC (differential interference contrast) images are shown on the right. (C) Control BiFC experiments with yeast lacking the p33 sequence. Yeast was grown under similar conditions and images were taken as in panel A–B. (D–E) BiFC analysis of interaction between Osh6p and the CIRV p36 in yeast cells. Confocal laser microscopy images show the mitochondrial localization of Ilv6-CFP or ER-localized Pho86-CFP. The merged images demonstrate the interaction between p36 and Osh6p and their partial co-localization with Ilv6-CFP and Pho86-CFP.