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. 2014 Oct 16;10(10):e1004388. doi: 10.1371/journal.ppat.1004388

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© 2014 Barajas et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Partial co-localization of TBSV BFP-tagged p33 replication protein with the YFP-AtPVA12 VAP protein in N. benthamiana cells. Expression of the above proteins from the 35S promoter was done after agro-infiltration into N. benthamiana leaves. Note the altered membrane structure in the subcellular area showing co-localization of BFP-p33 and YFP-AtPVA12 (portion of the image was enlarged at the bottom panel), which might be due to local membrane proliferation. (B) Co-localization of TBSV BFP-tagged p33 replication protein with the nYFP-AtPVA12 VAP and AtOrp3A-cYFP protein complexes in N. benthamiana cells. Expression of the above proteins from the 35S promoter was done after agro-infiltration into N. benthamiana leaves. Note that nYFP-AtPVA12 VAP and AtOrp3A-cYFP proteins were detected by BiFC. The subcellular areas (likely representing the ER membranes) where one viral and two cellular proteins are co-localized are marked with arrows. Control BiFC experiments were as in a previous paper (not shown) [56].