Skip to main content
. 2014 Oct 16;10(10):e1004426. doi: 10.1371/journal.ppat.1004426

Figure 4. SNP rs1143627 variant affects C/EBPβ and PU.1 binding to the IL1B promoter.

Figure 4

(A and B) Nuclear extracts were prepared PMA-differentiated U937 cells stimulated without (A) or with (B) Mtb lysate (20 µg/mL) for 24 h. Nuclear extracts were incubated with T-probe (lanes 1–4) or C-probe (lanes 5–8) in the absence or presence of unlabelled competitors and subjected to EMSA. (C) The relative abundance of complex 1 bound to T probe or C probe in (B) quantified by densitometry. Data are shown as the mean ± SEM, and differences between groups were compared with unpaired t tests. (D) Nuclear extracts prepared from (A) were incubated with T-probe in the absence (lane 2) or presence of antibodies against transcription factors including ZEB1, MEF2, C/EBPα, C/EBPβ, PU.1, TBP, and SP1 (lanes 4–10). Anti-C/EBPβ (lane 7) and anti-PU.1 (lane 8) supershift the DNA-protein complex 1. *, p<0.05.