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. 2014 Oct 16;10(10):e1004426. doi: 10.1371/journal.ppat.1004426

Figure 5. C/EBPB and PU.1 expression induced by Mtb temporally correlates with IL1B expression.

Figure 5

(A-D) SYBR Green-based real-time qPCR assay for the gene expression of IL1B (A), C/EBPB (B), PU.1 (C), and TBP (D) by differentiated U937 cells in response to Mtb lysate (20 µg/mL) or H37Ra infection (MOI = 10). The data are expressed as mRNA copy number relative to the house keeping gene GAPDH. Differences between groups were compared with the ANOVA/Newman-Keuls multiple comparison test. (E-G) Correlation analysis between the C/EBPB (E), PU.1 (F), or TBP (G) expression and IL1B expression. The coefficient r and P values are indicated. (H-K) Dynamic change of IL1B (H), C/EBPB (I), PU.1 (J) and TBP (K) expression by differentiated U937 upon stimulation with Mtb lysate (20 µg/mL). *, p<0.05; **, p<0.01.